Genetic transformation of Bacopa monnieri by wild type strains of Agrobacterium rhizogenes stimulates production of bacopa saponins in transformed calli and plants.

Genetic transformation of Bacopa monnieri by wild type strains of Agrobacterium rhizogenes stimulates production of bacopa saponins in transformed calli and plants.

Majumdar S, Garai S, Jha S.2011plant cell rep30:941-954

The major advantage of this approach is the rapidity and technical simplicity of A. rhizogenes transformation. Instead of the 4-6 months-time required to regenerate transgenic plants following A. tumefaciens-based transformation (see chapter “Agrobacterium tumefaciens-mediated transformation”), production of Ri T-DNA transgenic roots takes only a few weeks. Thus, this technique is particularly well adapted to RNAi-based approaches to study gene function (Limpens et al. 2004), performing functional promoter analyses (Boisson-Dernier et al. 2005) and as a means for rapidly evaluating the complementation of plant mutants withcandidate genes (e.g. Endre et al. 2002; Levy et al. 2004), when the genetic determinant of the corresponding mutation is root-determined.